67 research outputs found

    The Hepatitis E Virus Polyproline Region Is Involved in Viral Adaptation

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    Genomes of hepatitis E virus (HEV), rubivirus and cutthroat virus (CTV) contain a region of high proline density and low amino acid (aa) complexity, named the polyproline region (PPR). In HEV genotypes 1, 3 and 4, it is the only region within the non-structural open reading frame (ORF1) with positive selection (4–10 codons with dN/dS>1). This region has the highest density of sites with homoplasy values >0.5. Genotypes 3 and 4 show ∼3-fold increase in homoplastic density (HD) in the PPR compared to any other region in ORF1, genotype 1 does not exhibit significant HD (p<0.0001). PPR sequence divergence was found to be 2-fold greater for HEV genotypes 3 and 4 than for genotype 1. The data suggest the PPR plays an important role in host-range adaptation. Although the PPR appears to be hypervariable and homoplastic, it retains as much phylogenetic signal as any other similar sized region in the ORF1, indicating that convergent evolution operates within the major HEV phylogenetic lineages. Analyses of sequence-based secondary structure and the tertiary structure identify PPR as an intrinsically disordered region (IDR), implicating its role in regulation of replication. The identified propensity for the disorder-to-order state transitions indicates the PPR is involved in protein-protein interactions. Furthermore, the PPR of all four HEV genotypes contains seven putative linear binding motifs for ligands involved in the regulation of a wide number of cellular signaling processes. Structure-based analysis of possible molecular functions of these motifs showed the PPR is prone to bind a wide variety of ligands. Collectively, these data suggest a role for the PPR in HEV adaptation. Particularly as an IDR, the PPR likely contributes to fine tuning of viral replication through protein-protein interactions and should be considered as a target for development of novel anti-viral drugs

    Technology and the Era of the Mass Army

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    Design issues for grid-connected photovoltaic systems

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    Dissertation made openly available per email from author, 2/17/2017Ph.D.Ajeet Rohatg

    Incorporating Advanced Aircraft Technologies into an Aeronautical Engineering Technology Curriculum

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    Researchers in the Aeronautical Engineering Technology program at Purdue University are exploring innovative ways to introduce and integrate aircraft maintenance data from their advanced training fleet of networked aircraft into an undergraduate Aviation curriculum. This report describes a work in progress toward that goal. This initiative will better prepare students for an industry where synthetic process visualization, drag and drop planning screens and ‘‘smart’’ personal computing device applications play a significant role in problem solving and daily aircraft operations. The goal is to equip students at all levels of the curriculum with awareness and modern methods of process visualization, troubleshooting and research using modern, networked air vehicles
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